不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析

doi:10.11843/j.issn.0366-6964.2017.08.018

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (8): 1529-1534.doi: 10.11843/j.issn.0366-6964.2017.08.018

不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析

营凡^1,2, 李鹏², 邢思远², 李庆贺², 郑麦青², 刘冉冉², 崔焕先², 文杰², 马友记^1*, 赵桂苹^2*

1. 甘肃农业大学动物科学技术学院, 兰州 730070;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2017-02-20 出版日期:2017-08-23 发布日期:2017-08-23
通讯作者: 赵桂苹,女,研究员,E-mail:zhaoguiping@126.com;马友记,男,教授,主要从事动物遗传育种与繁殖研究,E-mail:yjma@gsau.edu.cn
作者简介:营凡(1990-),男,安徽宿州人,硕士,主要从事家禽遗传育种研究,E-mail:18697582881@163.com
基金资助:
中国农业科学院科技创新工程（ASTIP-IAS04）；国家科技支撑项目（2015BAD03B03）；国家自然科学基金（31572393）

The Analysis of the Difference of Salmonella Resistance between High and Low Heterophil/Lymphocyte Ratio in Chickens

YING Fan^1,2, LI Peng², XING Si-yuan², LI Qing-he², ZHENG Mai-qing², LIU Ran-ran², CUI Huan-xian², WEN Jie², MA You-ji^1*, ZHAO Gui-ping^2*

1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2017-02-20 Online:2017-08-23 Published:2017-08-23

摘要/Abstract

摘要：

旨在观察雏鸡异嗜性粒细胞与淋巴细胞比值（heterophil/lymphocyte，H/L）的高低对肠炎沙门菌免疫应答反应的差异，证明低H/L值的群体具有更优的抗性。分别选用京星黄鸡H系做亲本，该系已完成H/L选择8个世代，选择比值高低不同的公母鸡分组，按照公母比例1∶3组建半同胞家系。后代按亲本比值高低分为HG组（High H/L Group）和LG组（Low H/L Group），于7日龄口服肠炎沙门菌感染，感染后1、2、4、7、13 d（days post infection，dpi）进行死亡率、组织载菌量和血清介质指标的统计和检测。结果显示，感染沙门菌后，LG组的死亡率（16.7%）低于HG组（22.9%）；LG组肝组织载菌量在1、2、4、7 dpi显著低于HG组（P<0.05），13 dpi时差异不显著。LG组血清介质白细胞介素6（interleukin-6，IL-6）的浓度在1、2和4 dpi显著高于HG组（P<0.05），LG组血清肿瘤坏死因子α（tumor necrosis factor-α，TNF-α）和白三烯B4（leukotriene B4，LTB-4）的浓度在1和2 dpi显著高于HG组（P<0.05）。具有低H/L值的雏鸡机体对肠炎沙门菌感染的免疫应答反应更强，有更好的沙门菌抗性。

Abstract:

This study was conducted to reveal the difference of immune response upon Salmonella enteritidis (SE) infection between chickens with different Heterophil and Lymphocyte ratio, and trying to prove that chicken flocks of low H/L values has better resistance to Salmonella. Chicken with high and low H/L ratio were selected as parents, and the offspring were divided as High H/L Group and Low H/L Group (HG and LG). The ratio of male and female is 1:3. The chicks were challenged with SE at 7 days old. Liver, cecum and blood serum were collected at 1, 2, 4, 7 and 13 dpi (days post infection). Our results showed that the mortality rate of LG (16.7%) was significantly lower than HG (22.9%). The CFU of SE in liver were significantly different between HG and LG (P<0.05) at 1, 2, 4 and 7 dpi. IL-6 in serum were remarkably higher in Low H/L group than in high group (P<0.05) at 1, 2 and 4 dpi. Low H/L chickens had more TNF-α and LTB-4 in serum than high H/L chickens (P<0.05) at 1, 2 dpi. Chicken with low H/L ratio might have stronger immune response against bacterial infection.

中图分类号:

S813.2

营凡, 李鹏, 邢思远, 李庆贺, 郑麦青, 刘冉冉, 崔焕先, 文杰, 马友记, 赵桂苹. 不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析[J]. 畜牧兽医学报, 2017, 48(8): 1529-1534.

YING Fan, LI Peng, XING Si-yuan, LI Qing-he, ZHENG Mai-qing, LIU Ran-ran, CUI Huan-xian, WEN Jie, MA You-ji, ZHAO Gui-ping. The Analysis of the Difference of Salmonella Resistance between High and Low Heterophil/Lymphocyte Ratio in Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1529-1534.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2017.08.018

https://www.xmsyxb.com/CN/Y2017/V48/I8/1529

参考文献

[1] REDMOND S B, CHUAMMITRI P, ANDREASEN C B, et al. Genetic control of chicken heterophil function in advanced intercross lines:associations with novel and with known Salmonella resistance loci and a likely mechanism for cell death in extracellular trap production[J]. Immunogenetics, 2011, 63(7):449-458.
[2] STABLER J G, MCCORMICK T W, POWELL K C, et al. Avian heterophils and monocytes:phagocytic and bactericidal activities against Salmonella enteritidis[J]. Vet Microbiol, 1994, 38(4):293-305.
[3] KOGUT M H, CHIANG H I, SWAGGERTY C L, et al. Gene expression analysis of toll-like receptor pathways in heterophils from genetic chicken lines that differ in their susceptibility to Salmonella enteri-tidis[J]. Front Genet, 2012, 3:121.
[4] AL-MURRANI W K, AL-RAWI I K, RAOF N M. Genetic resistance to Salmonella typhimurium in two lines of chickens selected as resistant and sensitive on the basis of heterophil/lymphocyte ratio[J]. Br Poult Sci, 2002, 43(4):501-507.
[5] VAN DIJK A, TERSTEEG-ZIJDERVELD M H G, TJEERDSMA-VAN BOKHOVEN J L M, et al. Chicken heterophils are recruited to the site of Salmonella infection and release antibacterial mature Cathelicidin-2 upon stimulation with LPS[J]. Mol Immunol, 2009, 46(7):1517-1526.
[6] KOGUT M H, IQBAL M, HE H Q, et al. Expression and function of Toll-like receptors in chicken heterophils[J]. Dev Comp Immunol, 2005, 29(9):791-807.
[7] 刘向萍,郭晓敏,李志腾,等. 狼山鸡NLRC5基因启动子区SNP及其在先天免疫中的效应分析[J]. 畜牧兽医学报, 2016, 47(1):41-47.
LIU X P,GUO X M,LI Z T,et al. SNPs of promoter region of NLRC5 gene and its genetic effect on innate immune traits in Langshan chicken breeds[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(1):41-47. (in Chinese)
[8] FOTOUH A, GAB-ALLAH M S, TANTAWY A A, et al. Alterations of blood components in broiler chicks experimentally infected with Salmonella gallinarum[J]. Glob Vet, 2014, 13(5):787-793.
[9] MASELLO J F, CHOCONI R G, HELMER M, et al. Do leucocytes reflect condition in nestling burrowing parrots Cyanoliseus patagonus in the wild?[J]. Comp Biochem Physiol A Mol Integr Physiol, 2009, 152(2):176-181.
[10] JAKUBAS D, WOJCZULANIS-JAKUBAS K, KOS'MICKA A. Factors affecting leucocyte profiles in the little auk, a small Arctic seabird[J]. J Ornithol, 2015, 156(1):101-111.
[11] 胡耀东. 鸡H/L和SRBC遗传选择及部分抗病性状全基因组关联分析[D]. 雅安:四川农业大学, 2012.
HU Y D. The analysis of genetic selection of disease-resistant trait (H/L and SRBC) in chicken and genome-wide association study for part of disease-resistant traits[D]. Ya'an:Sichuan Agricultural University, 2012. (in Chinese)
[12] CAMPO J L, DAVILA S G. Effect of photoperiod on heterophil to lymphocyte ratio and tonic immobility duration of chickens[J]. Poult Sci, 2002, 81(11):1637-1639.
[13] ONBA ?ILAR E E, YALÇIN S, TORLAK E, et al. Effects of early feed restriction on live performance, carcass characteristics, meat and liver composition, some blood parameters, heterophil-lymphocyte ratio, antibody production and tonic immobility duration[J]. Trop Anim Health Prod, 2009, 41(7):1513-1519.
[14] ÇOBAN Ö, LAÇIN E, GENÇ M. The effect of photoperiod length on performance parameters, carcass characteristics and heterophil/lymphocyte-ratio in broilers[J]. Kafkas Üniv Vet Fak Derg, 2014, 20(6):863-870.
[15] COTTER P F. An examination of the utility of heterophil-lymphocyte ratios in assessing stress of caged hens[J]. Poult Sci, 2015, 94(3):512-517.
[16] 凡文磊. 利用RNA-seq技术挖掘鸡肠炎沙门氏菌抗性相关功能基因[D]. 北京:中国农业科学院, 2015.
FAN W L. RNA-seq analysis revealed functional genes associated with resistance to Salmonella enterica in chicken[D]. Beijing:Chinese Academy of Agricultural Sciences, 2015. (in Chinese)
[17] KOGUT M H, HE H Q, GENOVESE K J. Bacterial toll-like receptor agonists induce sequential NF-κB-mediated leukotriene B4 and prostaglandin E2 production in chicken heterophils[J]. Vet Immunol Immunopathol, 2012, 145(1-2):159-170.
[18] 杨尧,周瑶, 郁彭, 等. 白三烯A4水解酶及其抑制药研究进展[J]. 医药导报, 2015, 34(2):225-227.
YANG Y, ZHOU Y, YU P, et al. Research progress of leukotriene A4 hydrolase and its inhibitors[J]. Herald of Medicine, 2015, 34(2):225-227. (in Chinese)
[19] BERTHELOT-HÉRAULT F, MOMPART F, ZYGMUNT M S, et al. Antibody responses in the serum and gut of chicken lines differing in cecal carriage of Salmonella enteritidis[J]. Vet Immunol Immunopathol, 2003, 96(1-2):43-52.
[20] 李建超. 鸡肠炎沙门氏菌感染早期免疫指标变化及全基因组甲基化研究[D]. 扬州:扬州大学, 2014.
LI J C. The changes of immune performance in early enteritis Salmonella infected chickens and genome-wide methylation regulation analysis[D]. Yangzhou:Yangzhou University, 2014. (in Chinese)
[21] GROSS W B, SIEGEL P B. Effects of initial and second periods of fasting on heterophil/lymphocyte ratios and body weight[J]. Avian Dis, 1986, 30(2):345-346.
[22] AL-MURRANI W K, KASSAB A, AL-SAM H Z, et al. Heterophil/lymphocyte ratio as a selection criterion for heat resistance in domestic fowls[J]. Br Poult Sci, 1997, 38(2):159-163.
[23] SWAGGERTY C L, PEVZNER I Y, KOGUT M H. Selection for pro-inflammatory mediators yields chickens with increased resistance against Salmonella enterica serovar enteritidis[J]. Poult Sci, 2014, 93(3):535-544.

不同异嗜性粒细胞与淋巴细胞比值雏鸡对肠炎沙门菌抗性差异的分析

The Analysis of the Difference of Salmonella Resistance between High and Low Heterophil/Lymphocyte Ratio in Chickens

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王元清, 王兢, 朱波, 陈燕, 徐凌洋, 王泽昭, 张路培, 高会江, 李俊雅, 高雪. 基因组选配研究及其在畜禽育种中的应用[J]. 畜牧兽医学报, 2024, 55(1): 1-10.
[2]	李桃桃, 金美林, 费晓娟, 王慧华, 陆健, 狄冉, 魏彩虹. Hox基因家族及其对动物脊椎形成的影响[J]. 畜牧兽医学报, 2022, 53(4): 999-1009.
[3]	刘悦, 薛翔澜, 李晓波, 蒋琳, 浦亚斌, 何晓红, 马月辉, 赵倩君. 染色质开放性与动物胚胎发育关系的研究进展[J]. 畜牧兽医学报, 2022, 53(3): 680-687.
[4]	袁泽湖, 葛玲, 李发弟, 乐祥鹏, 孙伟. 整合生物学先验信息的全基因组选择方法及其在家畜育种中的应用进展[J]. 畜牧兽医学报, 2021, 52(12): 3323-3334.
[5]	谢宁, 张凯艺, 阮进学, 陶聪, 吴添文, 王彦芳, 杨述林. Glut4突变调控脂肪重分布及肌纤维重塑的机制研究[J]. 畜牧兽医学报, 2021, 52(9): 2464-2474.
[6]	王卫振, 邓占钊, 辛国省, 蔡正云, 顾亚玲, 张娟. 环状RNA的生物学功能及其在家禽中的研究进展[J]. 畜牧兽医学报, 2021, 52(7): 1778-1788.
[7]	牛化欣, 常杰, 胡宗福, Wang Yuxi. 基于组学技术研究反刍动物瘤胃微生物及其代谢功能的进展[J]. 畜牧兽医学报, 2019, 50(6): 1113-1122.
[8]	盛中伟, 单艳菊, 束婧婷, 章明, 姬改革, 屠云洁, 范建华, 邹剑敏. CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析[J]. 畜牧兽医学报, 2017, 48(10): 1825-1832.
[9]	王杰, 刘璐, 刘杰, 赵桂苹, 刘冉冉, 郑麦青, 文杰. 基于通路分析方法诠释肉鸡体重性状全基因组关联研究[J]. 畜牧兽医学报, 2017, 48(5): 810-817.
[10]	金连丰，于广璞，孙霞，李庆章，高学军，崔英俊. 牛GHR基因与miR-139靶向关系的验证[J]. 畜牧兽医学报, 2016, 47(12): 2398-2404.
[11]	章杰，罗宗刚. 纯种与杂种猪肌肉生长相关分子表达的差异分析[J]. 畜牧兽医学报, 2016, 47(12): 2370-2378.
[12]	栾兆进，刘开东，贺建宁，程明，曲绪仙，柳楠. 绵羊FAM134B、PPARγ、HSL和FAS基因表达量及与肌内脂肪含量的关系[J]. 畜牧兽医学报, 2016, 47(12): 2379-2389.
[13]	亓利思，佟慧丽，李树峰,胡倩，严云勤. 牛结蛋白基因启动子的初步改造研究[J]. 畜牧兽医学报, 2016, 47(12): 2405-2413.
[14]	张进威，龙科任，王讯，李明洲，马继登. 环状RNA研究进展[J]. 畜牧兽医学报, 2016, 47(11): 2151-2158.
[15]	张秀秀，郭云涛，黄万龙，李嫒，苗向阳. 靶向MAPK信号通路调控脂肪细胞分化的microRNAs[J]. 畜牧兽医学报, 2016, 47(11): 2159-2166.